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perm buffer (10X Genomics)

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10X Genomics perm buffer
Perm Buffer, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm/buffer+perm/pm42106323-210-2-4
Average 86 stars, based on 1 article reviews

perm buffer - by Bioz Stars, 2026-09

86/100 stars

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Article Title: Coevolution of neoplastic and non-neoplastic reactive astrocyte states converges on mesenchymal-like and injury-response programs during murine glioblastoma progression and post-radiotherapy recurrence
Article Snippet: Fix & Perm Buffer (10x Genomics, PN-2000517).

Article Title: p16 Ink4a -Positive Hepatocytes Drive Liver Fibrosis Through Activation of LIFR Family Pathway.
Article Snippet: The tissue fragments were fixed in Fix & Perm Buffer (10X Genomics) containing 4% formaldehyde (Polysciences) at 4◦C with moderate agitation for 16–20 h. Fixation was stopped by adding 1.25 m glycine, and the fragments were washed with tissue resuspension buffer (0.496X PBS (Nacalai Tesque), 50 m m Tris-HCl pH 8.0, 0.02% (w/v) BSA (Sigma), and RNase inhibitors (TOYOBO).

Incubation:

Article Title: Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatos.
Article Snippet: .. Fix/Perm buffer (10x Genomics, cat. no. PN-2000517), 4% formaldehyde in water) and then incubated overnight at 4 °C. ..

Article Title: Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatos
Article Snippet: .. Fix/Perm buffer (10x Genomics, cat. no. PN-2000517), 4% formaldehyde in water) and then incubated overnight at 4 °C. ..

Article Title: Oncogenic and tumor-suppressive forces converge on a progenitor-orchestrated niche to shape early tumorigenesis
Article Snippet: .. Nuclei were then resuspended in 1 mL of Fixation Buffer (4% formaldehyde in 1× Fix & Perm Buffer, 10x Genomics, PN-2000517) and incubated at 4°C for 16–24 h. To stop the fixation, nuclei were centrifuged at 850 g for 5 min at room temperature and quenched with 1 mL of Quenching Buffer (1× Quench Buffer, 10x Genomics, PN-2000516). ..

Formalin-fixed Paraffin-Embedded:

Article Title: Quantitative dissection of the metastatic cascade at single colony resolution
Article Snippet: Formalin-fixed paraffin-embedded (FFPE) blocks from metastases bearing C57BL/6 mouse liver and lung were sectioned at 5 μm and mounted onto Xenium slides following a modified version of the FFPE Tissue Preparation Guide (10x Genomics, CG000578, Rev D). .. Slides were deparaffinized with sequential xylene and ethanol washes, followed by decrosslinking using the FFPE Tissue Enhancer and diluted Perm Enzyme B (10x Genomics, CG000580, Rev E). ..

Article Title: Cellular senescence in human liver under normal aging and cancer
Article Snippet: FFPE passed screening were sectioned at 5 μm and mounted onto Xenium slides following a modified version of the FFPE Tissue Preparation Guide (10x Genomics, CG000578 , Rev D). .. Slides were deparaffinized with sequential xylene and ethanol washes, followed by decrosslinking using the FFPE Tissue Enhancer and diluted Perm Enzyme B (10x Genomics, CG000580 , Rev E). ..

Hybridization:

Article Title: Catecholaminergic neurons boost fibroblast osteogenic activity in keloid.
Article Snippet: .. Fix & Perm Buffer (10X Genomics cat. PN-2000517) at 4°C for 20 h. Afterwards, the fixation was quenched, and the cells were stored at −80°C until probe hybridization. .. Chromium Fixed RNA Kit, Human Transcriptome (10X Genomics cat. PN-1000474) was used for hybridization and library preparation.